{"id": "package:0031ba6c-c746-482d-93c1-5b7550cfc2dd", "name": "SPARC_BAT92_Dorsal_Upper_Spine.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/0031ba6c-c746-482d-93c1-5b7550cfc2dd", "size": 70735600, "created_time": "2020-01-23T16:04:57,826953Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "42da8e9ec6221cdef138ec0688c583b1384c0d6986516124fe1514734d5ceee1", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/primary/sub-iBAT92/TIFF Images/SPARC_BAT92_Dorsal_Upper_Spine.tif"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}