{"id": "package:0bd9b99a-657d-4dc3-823c-ec611533a00c", "name": "013-1.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/0bd9b99a-657d-4dc3-823c-ec611533a00c", "size": 219498257, "created_time": "2022-03-28T14:31:18,653641Z", "updated_time": "2024-12-20T14:15:03,567042Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "68fe2aceaedace571c573ce9610ed8723c018f3e652d521c1b1627e61ba06068", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/225/files/derivative/sub-VNS-013/sam-013-1/013-1.tif"}, "region": "us-east-1"}], "dataset": {"id": "225", "doi": "DOI:10.26275/rt8g-gu2v", "title": "Ultrastructural analysis of human vagus nerve", "description": "Ultrastructural studies of cervical and sub-diaphragmatic human vagus nerve segments", "abstract": "The mechanisms of action for vagus nerve stimulation (VNS) are not known, and high-resolution anatomical mapping of the human vagus nerve is needed to better understand its functional organization. Electron microscopy (EM) is required for the detection of both myelinated and unmyelinated axons. Intact human vagus nerve samples were procured intra-operatively from deceased organ donors, and tissues were immediately immersion fixed and processed for EM. Digital segmentation of myelinated and unmyelinated axons was perforemd for determination of fiber size and myelination. We conclude that vagal nerve biopsies from organ donors represent a new source of nervous tissues for combined light microscopy (LM) and EM studies that allows for excellent ultrastructure and detailed analysis of both myelinated and unmyelinated nerve fibers."}}