{"id": "package:1134e85b-3cfc-4b85-97e2-459859e4e501", "name": "slide-23 Mouse-14 SP 594 Duodenum.jp2", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/1134e85b-3cfc-4b85-97e2-459859e4e501", "size": 10797058, "created_time": "2024-02-16T04:55:29,745190Z", "updated_time": "2024-02-16T04:59:56,283032Z", "version": "1", "mime_type": "image/jp2", "checksums": [{"checksum": "1ba821c845434a576f2fc7a32ec8b50a", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/385/files/derivative/sub-14/sam-SP-mouse-APD-14-cross-section/slide-23 Mouse-14 SP 594 Duodenum.jp2"}, "region": "us-east-1"}], "dataset": {"id": "385", "doi": "DOI:10.26275/1ito-vvqo", "title": "Substance P-immunoreactive axons in the antrum-pylorus-duodenum of mice", "description": "Immunohistochemistry revealed the morphology and distribution of nociceptor substance P-immunoreactive (SP-IR) axons and terminals in the muscular, submucosal, and mucosal layers of the antrum, pylorus, and duodenum regions of the mice.", "abstract": "This work provides a comprehensive view of the distribution and morphology of SP-IR axons in all layers of APD at single-cell/axon/varicosity resolution. These data will establish a foundation for functional mapping of the nociceptive innervation of the GI tract and its pathological remodeling in gastrointestinal diseases and will be used to create a 3-D atlas of the SP-IR innervation of the APD region. This dataset contains montages of SP-IR axons and terminals in the ADP sections of 5 animals (sub-12, sub-14, sub-15, sub-16, sub-20). Each montage is a maximum projection image that is stitched from multiple single section images and is saved as .tif. We found that: 1) SP-IR fibers innervated all layers, including the longitudinal/circular muscles, myenteric ganglia, submucosa, submucosal ganglia, muscularis mucosa, and mucosal epithelium. Many SP-IR axons were also vesicular acetylcholine transporter-IR (VAChT, parasympathetic marker). 2) In the muscular layers, SP-IR varicose axons densely innervated the smooth muscles and formed heavy terminals which encircled numerous individual myenteric neurons. 3) In the submucosa, SP-IR axons innervated blood vessels and submucosal ganglia and formed a network in duodenal Brunner’s glands.4) In the mucosa, SP-IR axon bundles were found in the muscularis mucosa at the base of mucosa. Some SP-IR axons entered the gastric subepithelium and duodenal villi. 5) SP-IR axon density varied across the layers of the APD regions: density in the muscles was much higher than in the submucosa and mucosa. 6) The muscular wall of the antrum and duodenum showed a higher density than the pyloric sphincter."}}