{"id": "package:19d4ee46-3053-432c-929b-653c6ee82fc6", "name": "WGA dC mucosa M274 20XZ 220126_220812_24.jpx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/19d4ee46-3053-432c-929b-653c6ee82fc6", "size": 1213393, "created_time": "2024-01-17T01:57:35,239955Z", "updated_time": "2024-01-17T01:59:00,297855Z", "version": "1", "mime_type": "image/jpx", "checksums": [{"checksum": "2f7824ce7f1a45eede54488155ad3988", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/387/files/derivative/sub-274/sam-M274/WGA dC mucosa M274 20XZ 220126_220812_24.jpx"}, "region": "us-east-1"}], "dataset": {"id": "387", "doi": "DOI:10.26275/hipp-yfnn", "title": "Vasculature in mouse colon and relationship with enteric nervous system glia and macrophages", "description": "This dataset contains microscopic images examining vascular structures in the mouse colon, yielding 3D images.", "abstract": "To study distribution, morphology and innervation of vasculature in different mouse colonic segments and layers, as well as spatial relationships of the vasculature with the enteric plexuses, glia and macrophage. Vascular structures in the mouse colon were scanned by microscopy, and 3D images were acquired. The microscopic images include: (1) The blood vessels and branches in the submucosa and connections to the capillary networks in the mucosa and muscularis externa. (2) The capillary networks in the mucosa, anastomosed rings like honeycombs at the orifices of mucosa crypts, and the capillary rings at the base of crypts. (3) Microvessels in the muscularis externa. (4) Microvessels in the circular smooth muscle layer of the proximal colon. (4) PGP9.5-, tyrosine hydroxylase (TH)-, and calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers distributed along the vessels in the submucosa. (5) In the mucosa, PGP9.5-, CGRP- and vasoactive intestinal peptide (VIP)-immunoreactive nerves terminated close to the capillaries. (6) Cells and processes labelled by S100B and glia fibrillary acidic protein (GPAP) distributed mainly in the lamina propria and lower portion of the mucosa. (7) Dense Iba1 immunoreactive macrophages closely adjacent to the mucosal capillary rings. (8) A few macrophages, but no glia in apposition to microvessels in the submucosa and muscularis external. In the mouse colon: 1) there are differences of vasculature between the proximal and distal colon associated with the colonic morphological features; 2) the colonic mucosa contains significantly more microvessels than the muscularis externa; (3) there were more CGRP and VIP nerve fibers found close to microvessels in the mucosa and submucosa than the muscle layers."}}