{"id": "package:297f4484-c7d3-4ec7-a6d6-b5d2fbe5864b", "name": "sub17068-sam1_DRG.csv", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/297f4484-c7d3-4ec7-a6d6-b5d2fbe5864b", "size": 953, "created_time": "2019-12-30T02:51:42,817682Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "text/csv", "checksums": [{"checksum": "7f150f21eae2695df70ebb32fea7063d29541443027a1404252ce8f31c99e6ee", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/106/files/primary/sub17068/sub17068-sam1_DRG.csv"}, "region": "us-east-1"}], "dataset": {"id": "106", "doi": "DOI:10.26275/gdot-t59p", "title": "Immunohistochemical classification of sensory and autonomic neurons projecting to the lower urinary tract in rats", "description": "Quantitative immunohistochemical phenotyping of peripheral sacral sensory and autonomic postganglionic neurons innervating different regions of the lower urinary tract in male and female rats.", "abstract": "**Study purpose:** The aim of our study was to quantify functionally distinct classes of sensory and autonomic neurons that innervate the bladder body, bladder trigone, and proximal urethra, in adult male and female Sprague-Dawley rats.\n\n**Data collection:** Microinjection of retrograde tracers (Fast Blue, FluoroGold) identified sensory and autonomic neurons projecting to each region, which were then analyzed using immunofluorescence. LUT dorsal root ganglion (DRG) neurons in spinal levels L6 and S1 were quantified after stratifying into nociceptive (Trpv1) and myelinated (NF200) functional classes, then further classified by their expression of peptides, sodium channels or calcium-binding proteins (n=6). Quantitation of immunohistochemically classified pelvic ganglion (PG) neurons was performed for autonomic projections to each LUT region (n=6). \n\n**Primary conclusion:** Integration of this data will enable the development of a ganglion-organ connectome for sensory and autonomic innervation of three LUT regions in male and female rats."}}