{"id": "package:2dbf0197-981e-4e5e-b5fb-eff0157a9250", "name": "RM2 rat IVS CGRP 488 20x-05_AcquisitionBlock8_pt8-ApoTome deconvolution-39.czi", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/2dbf0197-981e-4e5e-b5fb-eff0157a9250", "size": 350100704, "created_time": "2024-01-09T17:50:12,389971Z", "updated_time": "2024-01-09T18:43:28,412028Z", "version": "1", "mime_type": "image/x.vnd.zeiss.czi", "checksums": [{"checksum": "6290f291add4a7df0371171ba473d28f068755bc4df564f2585328165b4fd7cc", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/373/files/source/sub-RM2/sam-5/montage/RM2 rat IVS CGRP 488 20x-05_AcquisitionBlock8_pt8-ApoTome deconvolution-39.czi"}, "region": "us-east-1"}], "dataset": {"id": "373", "doi": "DOI:10.26275/86ve-meck", "title": "Distribution and morphology of calcitonin gene-related peptide (CGRP) innervation in flat mounts of whole rat atria and ventricles", "description": "The sensory (nociceptive) innervation of the whole rat heart (including the atria and ventricles) was immunohistochemically labeled for CGRP. Moreover, high-quality images of the whole tissue were acquired at the single-cell/axon/varicosity resolution.", "abstract": "Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. To address this, we prepared whole rat heart tissues as flat-mounts, labeled them for CGRP, and acquired high quality images of the whole tissues. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls and the walls of the great vessels including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and interventricular septum."}}