{"id": "package:36dd472b-5f93-439c-8812-32f92fae6769", "name": "Light_Sheet_Protocol_Protocol.io.docx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/36dd472b-5f93-439c-8812-32f92fae6769", "size": 404777, "created_time": "2019-12-10T21:24:37,854973Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "checksums": [{"checksum": "54d85d48d71efd35559919dd72cffbc75b8666c9e9b7430ef080c77707c9d58d", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/protocol/Light_Sheet_Protocol_Protocol.io.docx"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}