{"id": "package:4239e0ea-72c3-443d-859f-fb049aaefa32", "name": "20191031M1_tC_SMP_rpChAT_Hu_20x.lsm.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/4239e0ea-72c3-443d-859f-fb049aaefa32", "size": 53492018, "created_time": "2021-05-27T16:18:58,516265Z", "updated_time": "2021-05-27T16:19:09,132693Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "996fed5a349d3caab30ed2100105370440f8177a1d66784de1cbedd7edae5195", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/158/files/derivative/sub-20191031M1/sam-rpChAT_Hu_M1/20191031M1_tC_SMP_rpChAT_Hu_20x.lsm.tif"}, "region": "us-east-1"}], "dataset": {"id": "158", "doi": "DOI:10.26275/i7dl-58h1", "title": "Antibodies tested in the colon – Mouse", "description": "List of antibodies that were tested in the mouse colon. Information on source, species, dilution, and whether the test was performed on whole colonic wall with CLARITY, or whole mount preparations of submucusal and myenteric plexus.", "abstract": "Study purpose: To establish a table listing antibodies that we tested and characterized to label enteric neurons and/or nerve fibers in the mouse colon. The table will serve as reference to assess the suitability of antibodies to be used in immunostaining of mouse colon enteric nervous system.    \nData collection: the murine colon samples were prepared as (1) whole mounts of the submucosal layer, and of myenteric and longitudinal muscle layers, and (2) whole colonic wall cleared by passive CLARITY technique (PACT). Immunofluorescent methods were used using antibodies to label the neurons by pan-neuronal markers, neuropeptides, classical neurotransmitter-synthesizing enzymes. A few antibodies for non-neuronal markers that play important role in motility and inflammatory response were also tested, including the macrophage markers, glial cells and interstitial cells of Cajal (ICC) markers. Photomicrographs were acquired in confocal microscopes. Remarks and notes about the antibodies are briefly described and some representative figures are assembled in a file for preview of immunofluorescent staining. \nPrimary conclusion: The dataset provides information of primary antibodies tested in the mouse colon, which has been less studied compared with the small intestine of guinea pig and rats. In addition, the labeling of colonic submucosal layers or whole wall processed with CLARITY were rarely reported before."}}