{"id": "package:44430e00-df1f-4bf0-96b8-d8ac40be6819", "name": "sam-08-deriv.xml", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/44430e00-df1f-4bf0-96b8-d8ac40be6819", "size": 670696, "created_time": "2024-06-27T06:26:38,887025Z", "updated_time": "2024-06-27T06:28:30,115210Z", "version": "1", "mime_type": "text/xml", "checksums": [{"checksum": "13f2904ba5483b4519ad8ea967e36757", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/402/files/derivative/sub-03/sam-08/sam-08-deriv.xml"}, "region": "us-east-1"}], "dataset": {"id": "402", "doi": "DOI:10.26275/2nyl-vpb8", "title": "3D segmentation of myelinated afferents of the rat bladder in the pelvic nerve", "description": "Using a combination of retrograde tracing from the bladder, an adeno-associated virus that preferentially labels myelinated afferents in rats, and a robust immunolabel for paranodes, myelinated bladder afferents were traced in the pelvic nerve of rats.", "abstract": "Myelinated visceral afferents of the urinary bladder are critical for successful micturition. Most of these Aδ-class afferents reach the lumbosacral spinal cord via the pelvic nerves. Using a combination of retrograde tracing from the bladder (cholera toxin subunit B), and preferential sparse labelling of myelinated afferents (AAV-PHP.S), the myelinated afferents of the bladder could be visualized in Sprague-Dawley rats. Tracing these myelinated axons in Neurolucida 360, a rich dataset of myelinated bladder afferents was created, with nodes of Ranvier identified along their length using the paranode marker, neurofascin. This dataset contains wholemount image stacks of pelvic nerve fascicles from male and female Sprague-Dawley rats. These rats were intravenously injected with AAV-PHP.S which preferentially transfects peripheral neurons, notably myelinated afferents in rats. In addition, the bladder was microinjected with cholera toxin subunit B (CTB) to retrogradely label bladder-innervating afferents. Major pelvic ganglia were removed and immunolabelled with pelvic nerve attached for AAV-PHP.S' fluorophore (TdTomato), CTB and neurofascin (paranode marker). The pelvic nerves were then sub-dissected from the major pelvic ganglia and fascicles containing AAV-PHP.S and CTB were imaged at 40x magnification on the LSM900 (Carl Zeiss Microscopes). The myelinated afferents innervating the bladder of the rat are thin in diameter, similar to that of A-delta class afferent fibers. Internode distance (i.e. length between nodes) varied greatly between axons and was unrelated to axon diameter."}}