{"id": "package:4898eaef-3b0d-48e5-a010-614c2e1ca1c4", "name": "7.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/4898eaef-3b0d-48e5-a010-614c2e1ca1c4", "size": 3150620, "created_time": "2024-10-31T18:31:38,018120Z", "updated_time": "2024-10-31T18:36:52,357796Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "d45cfcd0e2f0eb17f5d7c8999743f654", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/421/files/primary/sub-unknown-1/sam-axon-1/7.tif"}, "region": "us-east-1"}], "dataset": {"id": "421", "doi": "DOI:10.26275/jjd7-yomj", "title": "Anterograde tracing of spinal afferent innervation in flat-mounts of the rat atria", "description": "This dataset displays the digitally traced spinal afferent axons in whole flat-mounts of the rat left and right atria using tracer Dextran Biotin injection into the left DRG C8-T3.", "abstract": "The spinal afferent innervation of the heart helps to regulate cardiac functions by sending sensory information through the dorsal root ganglia (DRG) to the brain. However, the distribution and morphology of spinal afferent axons in the heart have not been well characterized. In this study, we injected anterograde tracer Dextran Biotin (DB) into the left DRG C8-T3 to characterize spinal afferent axons and their terminal structures in flat-mounts of the whole rat atria. This dataset displays the digitally traced spinal afferent axons and their terminal structures in flat-mounts of the whole Sprague-Dawley rat left and right atria using tracer Dextran Biotin into the left DRG C8-T3. Our results showed that DB-labeled axons entered the left precaval vein and projected to the left and right atria, with predominant projection in the left atrial wall. DB-labeled varicose axons were observed in different layers, mostly in the epicardium and myocardium, but much less in the endocardium. In those layers, these spinal afferent axons branched out into simple to complex terminal arborizations, forming close appositions with cardiac muscles, intrinsic cardiac ganglia, blood vessels, and fat tissue. This work, for the first time, characterized cardiac spinal afferent distribution of the rat atria using anterograde tracing, which will provide the foundation for future studies of topographical cardiac spinal afferent innervation and remodeling in heart disease models."}}