{"id": "package:4b8cbfb0-c916-4206-9f28-2dda1a91f974", "name": "Snap-305.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/4b8cbfb0-c916-4206-9f28-2dda1a91f974", "size": 2456282, "created_time": "2021-08-31T15:28:11,295126Z", "updated_time": "2021-08-31T15:28:11,869618Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "9c7f886d8b649dc8429a234200608beba92677a2d3b81f8c52e6bc5444fd3499", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/354/files/primary/sub-216/sam-216-tdTomato-flox-mouse-uni-PVA-rAAV-retro-Cre-injection-TH-Rb-Jax-FITC/Snap-305.tif"}, "region": "us-east-1"}], "dataset": {"id": "354", "doi": "DOI:10.26275/ahqc-ldar", "title": "Identification of lung innervating sensory neurons and their target specificity in mouse (3)", "description": "Immunostaining and imaging of Cre reporter lines in mouse lung lobes", "abstract": "Known as the gas exchange organ, the lung is also critical for responding to the aerosol environment in part through interaction with the nervous system. The diversity and specificity of lung innervating neurons remain poorly understood. Our goal wasto map the innervation pattern of the mouse lung and projections from the nodose ganglia. This dataset contains Zeiss LightSheet immunofluorescence imaging of neuronal markers in mouse lung tissue. During our SPARC project, we interrogated the cell body location, molecular signature and projection pattern of lung innervating sensory neurons in the mouse. Retrograde tracing from the lung coupled with whole tissue clearing highlighted neurons primarily in the vagal ganglia. Centrally, they project specifically to the nucleus of the solitary tract in the brainstem. Peripherally, they enter the lung alongside branching airways. Labeling of nociceptor Trpv1+ versus peptidergic Tac1+ vagal neurons showed shared and distinct terminal morphology and targeting to airway smooth muscles, vasculature including lymphatics, and alveoli. Notably, a small population of Calb1+ neurons preferentially innervate pulmonary neuroendocrine cells, a demonstrated airway sensor population. This atlas of lung innervating neurons serves as a foundation for understanding their function in lung.\nDuring our SPARC project, we interrogated the cell body location, molecular signature and projection pattern of lung innervating sensory neurons in the mouse. Retrograde tracing from the lung coupled with whole tissue clearing highlighted neurons primarily in the vagal ganglia. Centrally, they project specifically to the nucleus of the solitary tract in the brainstem. Peripherally, they enter the lung alongside branching airways. Labeling of nociceptor Trpv1+ versus peptidergic Tac1+ vagal neurons showed shared and distinct terminal morphology and targeting to airway smooth muscles, vasculature including lymphatics, and alveoli. Notably, a small population of Calb1+ neurons preferentially innervate pulmonary neuroendocrine cells, a demonstrated airway sensor population. This atlas of lung innervating neurons serves as a foundation for understanding their function in lung.\nDuring our SPARC project, we interrogated the cell body location, molecular signature and projection pattern of lung innervating sensory neurons in the mouse. Retrograde tracing from the lung coupled with whole tissue clearing highlighted neurons primarily in the vagal ganglia. Centrally, they project specifically to the nucleus of the solitary tract in the brainstem. Peripherally, they enter the lung alongside branching airways. Labeling of nociceptor Trpv1+ versus peptidergic Tac1+ vagal neurons showed shared and distinct terminal morphology and targeting to airway smooth muscles, vasculature including lymphatics, and alveoli. Notably, a small population of Calb1+ neurons preferentially innervate pulmonary neuroendocrine cells, a demonstrated airway sensor population. This atlas of lung innervating neurons serves as a foundation for understanding their function in lung."}}