{"id": "package:4f3985bd-dfc9-43ad-840f-86afcd64ea02", "name": "sam-fund48_ses-hu594_bg10-SMPsubset-max.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/4f3985bd-dfc9-43ad-840f-86afcd64ea02", "size": 18294155, "created_time": "2021-03-17T13:19:03,444665Z", "updated_time": "2022-01-14T20:48:57,096132Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "c9c95fcf69ddb59259bd1c094d83bbe97dc50f96937e0e30087a125a723b691b", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/389/files/primary/sub-rat48/sam-fund48/sam-fund48_ses-hu594_bg10-SMPsubset-max.tif"}, "region": "us-east-1"}], "dataset": {"id": "389", "doi": "DOI:10.26275/odx3-c5cv", "title": "Quantification of enteric ganglia in the three main regions of the rat stomach", "description": "This dataset contains images and analysis used to quantify the number of neurons per ganglia, size and density of myenteric and submucosal ganglia in the rat gastric fundus, corpus and antrum.", "abstract": "The aim of this study was to provide a quantitative account of the ganglionated plexuses of the enteric nervous system within the stomach of the rat. Enteric ganglia were visualized using immunohistochemistry for markers of neuronal cell bodies in cleared wholemounts of the stomach wall harvested from Sprague Dawley rats in a non-fasted state. Confocal microscopy was used to produce 3 dimensional images of the enteric ganglia in large regions of the stomach wall from the fundus, corpus and antrum. An algorithm was developed to segment the ganglia in three dimensions and the sizes, volumes and densities of ganglia as well as the numbers of neurons they contain were quantified and compared between regions. Myenteric ganglia are prominent and numerous whereas submucous ganglia are small and sparse. Myenteric ganglia in particular are larger and more sparse in the antrum and corpus than they are in the fundus. There are over 400,000 neuronal cell bodies in the rat stomach."}}