{"id": "package:4fa8a744-218b-4ae6-8f17-4cf876b97f0b", "name": "sam-20190402-NW-cFos-egr1-01.czi", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/4fa8a744-218b-4ae6-8f17-4cf876b97f0b", "size": 68353440, "created_time": "2019-12-19T22:18:43,290116Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "image/x.vnd.zeiss.czi", "checksums": [{"checksum": "5984f09c824378c34237fe4fc5d2c181d665286e45a65696bca7a0368971543e", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/88/files/primary/sub-keastlab-r19-422/czi/sam-20190402-NW-cFos-egr1-01.czi"}, "region": "us-east-1"}], "dataset": {"id": "88", "doi": "DOI:10.26275/jg3k-z5qm", "title": "Immediate early gene (IEG) mapping of spinal cord neurons activated by cystometry induced micturition in rat", "description": "Spatial mapping of cystometry-activated neurons in the sacral spinal cord in awake male and female rats.", "abstract": "**Study purpose:**  This project will produce mesoscopic and microscopic functional anatomical maps of the sensory and autonomic (motor) neurons that regulate the lower urinary tract (LUT). We will fill knowledge gaps and overcome diverse technical roadblocks to develop maps that provide a critical foundation for modeling different LUT behaviors, understanding neuromodulatory mechanisms, and determining off-target effects of neuromodulation.\n\n**Data collection:** We will conduct this study in adult rats, the species where the peripheral and spinal circuitry of the LUT is defined in the most detail and, compared with mice, their larger size facilitates development of devices. The aims to be addressed in males and females are: (1) To map functionally distinct classes of sensory and autonomic neurons that innervate the bladder body, trigone, and proximal urethra. We will also map neurons that project to more than one of these regions (`cross-talk'). In addition to known functional markers of sensory and autonomic neural subtypes, we will use the latest outcomes of our RNA-seq analyses of retrogradely labeled bladder-projecting neurons to define and map new markers of functional subclasses. (2) To map the spinal targets of sensory neurons innervating the bladder body, trigone, and proximal urethra. Using viral tracing we will map and characterize second order sensory neurons in autonomic reflex and nociceptive pathways; we will also identify sites of convergence from two peripheral targets. (3) To map the spinal neurons activated by physiologically- or electrically-induced voiding in conscious rats.\n\n**Primary conclusion:** None Stated"}}