{"id": "package:5c49f025-c36d-4648-bc23-f9b5486add4c", "name": "iWAT36_VLS_SP5_5x_THch1_ChATch2_GFPch3.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/5c49f025-c36d-4648-bc23-f9b5486add4c", "size": 27726227818, "created_time": "2021-07-14T13:54:42,514079Z", "updated_time": "2021-07-14T14:18:13,109634Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "40657a9e51b3ee696a772f32d266dcb35b2ea3636e27cfdad8c5869b91451016", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT36/sam-iWAT36/iWAT36_VLS_SP5_5x_THch1_ChATch2_GFPch3.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}