{"id": "package:5f42509d-9b6f-4049-aeea-63a1d94342b9", "name": "19129008_.abf", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/5f42509d-9b6f-4049-aeea-63a1d94342b9", "size": 738304, "created_time": "2024-11-27T20:01:45,536723Z", "updated_time": "2024-11-27T20:01:55,644856Z", "version": "2", "mime_type": "application/x.vnd.axon-instruments.abf", "checksums": [{"checksum": "5d153f076dfee85c3b13369785999948", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://sparc-prod-aod-discover-publish50-use1/440/files/primary/Pig/Cellular Electrophsiology/Excitability Data/19129008_.abf"}, "region": "us-east-1"}], "dataset": {"id": "440", "doi": "DOI:10.26275/uldw-kfjq", "title": "Comparative specialization of intrinsic cardiac neurons in humans mice and pigs", "description": "Intrinsic cardiac neurons of mice pigs and humans", "abstract": "An understanding of the underlying cell structure and diversity among species is essential for developing an informed approach for neuromodulation strategies targeting epicardial neurons. Here, we present a structural and functional atlas of intrinsic cardiac neurons (ICNs) at macro-, meso- and nanoscales from three species: mice, pigs, and humans. Our focus was on clusters of epicardial neurons within the right atrial ganglionated plexus (RAGP), which contain neurons essential to the regulation of heart rate at the SAN. We took a multidisciplinary approach to complete a detailed cellular comparison of the structure and function of ICNs from mice, pigs, and humans. Immunohistochemistry of whole and sectioned ganglia, transmission electron microscopy, intracellular microelectrode recording and dye filling for quantitative morphometry were used to define the neurophysiology, histochemistry, and ultrastructure of these cells across species. Through our investigation, we identified both conserved and derived attributes of these cells among species. Adaptations in synaptic function, neuronal morphology, and neurochemistry revealed specificities in ICN structure and function across mammals, highlighting divergent specifications of human ICNs from those of mice and pigs. This study represents one of the first efforts to characterize human ICN membrane physiology."}}