{"id": "package:66ce310a-9d29-45f4-bcbb-fddb2511bf16", "name": "ventral-upper-spine_thumbnail.jpg", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/66ce310a-9d29-45f4-bcbb-fddb2511bf16", "size": 46726, "created_time": "2022-08-03T03:29:55,917136Z", "updated_time": "2022-08-03T03:29:56,628354Z", "version": "1", "mime_type": "image/jpeg", "checksums": [{"checksum": "ae13d9a9c7720c7f0df52ca47334091f24d6d50e6ad7d8064ef2b0d1d2653422", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/derivative/sub-iBAT3/AVI video files/ventral-upper-spine_thumbnail.jpg"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}