{"id": "package:685f71b1-10f7-4394-a973-3d441e667cd4", "name": "iWAT10_DUS_GFPch1_THch2_CD31ch3.ims", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/685f71b1-10f7-4394-a973-3d441e667cd4", "size": 2657684774, "created_time": "2019-12-10T21:53:21,517808Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.ms-ims", "checksums": [{"checksum": "c9276ebcfc3de210b97c7f27fe609fc7fc55343f1c55254b980dd347b8dede65", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/primary/sub-iWAT10/sam-iWAT10/iWAT10_DUS_GFPch1_THch2_CD31ch3.ims"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}