{"id": "package:688c6e6f-c155-48c3-8197-7e8f12e2b22d", "name": "iWAT22_VLS_GFPch1_THch2_CD31ch3.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/688c6e6f-c155-48c3-8197-7e8f12e2b22d", "size": 4757245176, "created_time": "2021-07-12T18:55:42,663381Z", "updated_time": "2021-07-12T19:00:26,866567Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "cdce0369fb19cd2de0079e514d8e0e78e387aee29479ff3ab1897cf41752d224", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT22/sam-iWAT22/iWAT22_VLS_GFPch1_THch2_CD31ch3.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}