{"id": "package:825b9c5c-efe7-42ef-8421-22a23d319217", "name": "manifest.xlsx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/825b9c5c-efe7-42ef-8421-22a23d319217", "size": 10502, "created_time": "2020-02-11T02:54:41,618064Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "checksums": [{"checksum": "82fdd3bb3ded1e1b029321b6d6b08603c4ebf825e85a307c6232c2640da0c5cb", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/90/files/primary/sub-898/manifest.xlsx"}, "region": "us-east-1"}], "dataset": {"id": "90", "doi": "DOI:10.26275/ilkm-9f8r", "title": "Morphometric analysis of the abdominal vagus nerve in rats", "description": "Determination of light and electron microscopy characteristics of the abdominal vagus nerve in rats.", "abstract": "Light microscopy images of cross sections of abdominal vagus nerve in rats. This dataset allows a detailed evaluation of nerve fiber populations that are crucial for the successful development of neuromodulation strategies used to repair a CNS/PNS injury or disease. Rat abdominal vagus nerve were embedded in plastic resin after perfusion and post fixation in 2% paraformaldehyde, 1.25% glutaraldehyde solution diluted in 0.1M PBS followed by cross sections that were stained with 1% toluidine blue.  The parameters investigated were: number of myelinated fibers; spatial distribution of myelinated fibers within the nerve structure; number of fascicles within the nerve; number of Schwann cell nuclei; number of blood vessels; area of fascicle; area of blood vessels; percentage of blood vessels and Schwann cell nuclei that occupies the fascicles; and morphometric analysis (myelinated fiber diameter; axon diameter; myelin thickness; and g-ratio). The histological images were captured with a Nikon® camera and the NIS Elements software and uploaded in .jp2 and .tiff formats. There are images of an overview of the entire nerve (10X), 100X magnification images, and a montage of the entire nerve in a higher magnification (100X) for both fascicles 1 and 2 for each animal (n=3), totaling 5.36GB of images."}}