{"id": "package:859eac8a-39b0-4e25-a93c-3d4a68f995e2", "name": "README.txt", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/859eac8a-39b0-4e25-a93c-3d4a68f995e2", "size": 208, "created_time": "2020-02-27T22:29:41,785023Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "text/plain", "checksums": [{"checksum": "f7288df13c8edb78cb65c9fc58044d9578927c3912d9179fd72100a771dbbdb8", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/derivative/sub-iBAT7/README.txt"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}