{"id": "package:86b332de-1700-4e45-a0bc-e2fce0c5e9e9", "name": "2018_09_19_0006_SR.abf", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/86b332de-1700-4e45-a0bc-e2fce0c5e9e9", "size": 1865216, "created_time": "2020-01-10T00:46:14,767006Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/x.vnd.axon-instruments.abf", "checksums": [{"checksum": "f00bcb7a62d6ab9f1256aed728ce5b3f342f883e8de9c04fc4d5fe76db491351", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/228/files/primary/sub-9/sam-2018-09-19-0006-SR/2018_09_19_0006_SR.abf"}, "region": "us-east-1"}], "dataset": {"id": "228", "doi": "DOI:10.26275/jsaw-2w1q", "title": "Sympathetic and parasympathetic effects on membrane currents in isolated pig ventricular myocytes", "description": "Patch clamp recording of L-type Ca2+ current and slow delayed rectifier K+ current responses to norepinephrine and acetylcholine.", "abstract": "Sympathetic and parasympathetic stimulation play an essential role in regulating the electrical properties of ventricular myocytes in the heart by modulating the activity of a number of different ion channels. Under normal conditions, changes in the activity of the depolarizing inward L-type Ca2+ current are carefully balanced with by changes in the activity of one or more repolarizing outward currents. This allows for changes in Ca2+ influx necessary for regulation of contraction, while preventing potentially arrhythmogenic changes in action potential duration.  However, exactly which ion channels are affected and their relative senstivity to sympathetic and parasympathetic activation varies from species to species. The pig is an excellent model for studying the effects of the autonomic nerve stimulation on cardiac function at the whole heart level. However, the ionic basis for ventircular myocyte responses has never been determined. The focus of this study was to characterize the effects of sympathetic (norepinephrine) and parasympathetic (acetylcholine) stimulation on the activity of ion channels found in isolated ventricular myocytes. Our goal initial goal was to characterize the effects of norepinephrine and acetylcholine on the L-type Ca2+ current and slow delayed rectifier K+ current using whole cell patch clamp techniques. This information is meant to be used in generating computational models that are able to accurately predict mechanisms contributing to autonomic effects on cardiac function in the pig heart."}}