{"id": "package:86ccba9f-bae9-43dd-b972-29d273f0e8dd", "name": "iWAT10_IML_GFPch1_THch2_CD31ch3.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/86ccba9f-bae9-43dd-b972-29d273f0e8dd", "size": 4427429390, "created_time": "2021-08-03T16:29:37,330508Z", "updated_time": "2021-08-03T16:35:47,017756Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "8e27f1623f1b845d53fcd6894fae09a28d60e9b216f188b819caaab3bac4f5e3", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT10/sam-iWAT10/iWAT10_IML_GFPch1_THch2_CD31ch3.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}