{"id": "package:8d3bc66c-05a7-4120-8816-8609de08f0e4", "name": "9 - 20210211 4033-p Prox huC 594 nNOS 488 ChAT 647 20x_SP4.jpx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/8d3bc66c-05a7-4120-8816-8609de08f0e4", "size": 16223885, "created_time": "2024-04-26T02:29:43,047857Z", "updated_time": "2024-04-26T02:34:21,241472Z", "version": "1", "mime_type": "image/jpx", "checksums": [{"checksum": "eee7740edcb38d7924066d9efba9d036", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/423/files/derivative/sub-4033-P/sam-HSCR1-proximal-margin/9 - 20210211 4033-p Prox huC 594 nNOS 488 ChAT 647 20x_SP4.jpx"}, "region": "us-east-1"}], "dataset": {"id": "423", "doi": "DOI:10.26275/whgt-j5vy", "title": "Three-dimensional imaging of the enteric nervous system in human pediatric colon reveals new features of Hirschsprung disease", "description": "Human pediatric colon enteric nervous system including Hirschsprung disease resections", "abstract": "This study was conducted to define normal human pediatric enteric nervous system anatomy in distal colon and to evaluate enteric nervous system anatomy in colon resected from children with Hirschsprung disease during pull-through surgery (a standard part of their clinical care). Human pediatric colon was cleared, stained with antibodies and imaged by confocal microscopy to visualize the enteric nervous system without tissue sectioning. The data provide 3-dimensional images of enteric nervous system anatomy. Tissue clearing and 3-dimensional imaging after immunohistochemistry provide much better information about enteric nervous system anatomy than traditional tissue sectioning. Human colon enteric nervous system anatomy changes throughout childhood so adult \"normal values\" should not be applied to children. The enteric nervous system often looks abnormal even in proximal margins of colon resected from children with Hirschsprung disease, with a wide range of structural variants between different children's resected colon. It is not yet known what anatomic features predict good outcomes after pull-through surgery for Hirschsprung disease."}}