{"id": "package:91c280ed-1c89-4d74-8256-238a3ff24bfd", "name": "iWAT_40_VLS_LS_ZF208_TH1Ch1_GFPCh2.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/91c280ed-1c89-4d74-8256-238a3ff24bfd", "size": 65508807426, "created_time": "2021-07-15T15:50:43,295483Z", "updated_time": "2021-07-15T19:31:28,546838Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "2bd41afe59a3ffa775e0b397be22a31c8558c37eecd6afd125aa40476f89e0e4", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT40/sam-iWAT40/iWAT_40_VLS_LS_ZF208_TH1Ch1_GFPCh2.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}