{"id": "package:9488efaf-957c-406e-8181-c18f577255d1", "name": "sub-rat07-sam-fiducialpointN-ses-massons trichrome.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/9488efaf-957c-406e-8181-c18f577255d1", "size": 30375964, "created_time": "2023-05-24T06:37:04,878792Z", "updated_time": "2023-05-24T06:37:24,762654Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "8e416de760e8ce3a3d39df1738fc71b8", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/367/files/derivative/sub-rat07/sam-07/sub-rat07-sam-fiducialpointN-ses-massons trichrome.tif"}, "region": "us-east-1"}], "dataset": {"id": "367", "doi": "DOI:10.26275/tlgo-dfke", "title": "Quantification of thickness of the gastric muscle in the rat stomach", "description": "This dataset contains images and analysis used to quantify the thickness of the mucosal and muscle layers of the rat stomach and to determine muscle orientations and relationships.", "abstract": "To measure the dimensions of the mucosal and muscle layers of the rat stomach and to determine relationships between the layers, in order to understand how these orientations influence the stomach function. Stomachs from adult Sprague-Dawley rats were collected, fixed and 15 fiducial points were located, these were distributed in all three regions (fundus, corpus and antrum) along the lesser and greater curvature. Full thickness preparations were collected from all points, sectioned at 5μm and stained with either haematoxylin and eosin (H&E) or Masson’s trichrome staining. At each site three measurements of each muscle layer were taken and averaged to provide the sample mean for that site for the individual rat. The average was taken for all equivalent fiducial points from at least 4 animals (2 males and 2 females). Stomachs were also collected to determine the directions and relationships of the muscle coats (at least 4 animals; 2 males and 2 females). These data show that the muscle layers in the stomach vary in thickness depending on their location and thus assumed roles in gastric function. These data are the first of its kind providing a detailed quantitative dataset concerning the organisation of the musculature of the rat stomach, which is expected to enhance modelling of gastric function and will be an important resource for future studies."}}