{"id": "package:9a3ee8d7-83f5-4ab9-af78-523c1997c4db", "name": "H2256_S_MP_Peripherin_MAX_VFN28-30_20X..jpg", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/9a3ee8d7-83f5-4ab9-af78-523c1997c4db", "size": 563267, "created_time": "2022-07-06T06:32:04,130377Z", "updated_time": "2022-07-06T06:32:04,633809Z", "version": "2", "mime_type": "image/jpeg", "checksums": [{"checksum": "300591d837a77bb10a4bf7b7ac52f3831035aa160e18b55c79222cdd37fedf58", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/290/files/primary/sub-H2256/sam-Peripherin-H2256/H2256_S_MP_Peripherin_MAX_VFN28-30_20X..jpg"}, "region": "us-east-1"}], "dataset": {"id": "290", "doi": "DOI:10.26275/puzi-xtm3", "title": "Antibodies tested in the colon – Human", "description": "List of antibodies that were tested in the human colon.", "abstract": "To establish a table listing antibodies that we tested and characterized to label enteric neurons and/or nerve fibers, enteric glial cells and interstitial cells of Cajal (ICC) in the human colon. The table will serve as reference to assess the suitability of antibodies to be used in immunostaining with dissected whole mount preparations of enteric plexus, CLARITY cleared intact samples and/or cryostat sections of human colon. Human colon samples were collected from operating theatres with prior informed consent of patients undergoing elective surgery, mostly for colonic carcinoma.  Specimens were obtained from ascending, transverse, descending or sigmoid colon, stretched and fixed in formaldehyde-based fixatives.  They were then either dissected as (1) whole mounts of the submucosal layer, (2) wholemounts of myenteric plexus with adhering longitudinal muscle, (3) whole colonic wall cleared by passive CLARITY technique (PACT), or (4) cryostat sections (8-10 µm).  Each of the antibodies listed in the table was applied to the tissue, followed by appropriate fluorescent labeled secondary antisera and their ability to distinguish selectively us neuronal subpopulations, enteric glial cells or interstitial cells of Cajal was determined.  In some cases, double, treble or quadruple immunostaining was used to determine the co-localization of different markers. Photomicrographs were acquired with confocal or epifluorescence microscopes. Remarks and notes about the antibodies are briefly summarized and some representative figures are assembled in a file for preview of immunofluorescent staining. The dataset provides information on primary antibodies tested in the human colon with an indication of the quality of staining that can be expected with each. In addition, the compatibility of immunolabeling with passive CLARITY technique has been assessed for some antibodies."}}