{"id": "package:9ffc56ef-f3cc-4a7d-8342-49eb67555378", "name": "iBAT_thumbnail.jpg", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/9ffc56ef-f3cc-4a7d-8342-49eb67555378", "size": 40266, "created_time": "2022-08-03T02:52:01,027441Z", "updated_time": "2022-08-03T02:52:01,564537Z", "version": "1", "mime_type": "image/jpeg", "checksums": [{"checksum": "c5936c5d3a0fd27a9b2fb980972f5f6a0e6ab9d0a7b0fc56ed14776d6564070f", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/derivative/sub-iBAT87/AVI video files/iBAT_thumbnail.jpg"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}