{"id": "package:a4e6e69b-6e1a-4485-a8db-d9ba09b8d9bb", "name": "manifest.xlsx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/a4e6e69b-6e1a-4485-a8db-d9ba09b8d9bb", "size": 11593, "created_time": "2022-08-03T03:04:41,877568Z", "updated_time": "2022-08-03T03:04:42,683897Z", "version": "1", "mime_type": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "checksums": [{"checksum": "3613931d0fc3ba771820dae305cc6d5208afa15ff77b4407df9b4baed8f0442b", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/derivative/sub-iBAT87/manifest.xlsx"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}