{"id": "package:a5893a97-deca-4745-bd76-43fefb409f85", "name": "AAV9-CG-IP-05.ims", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/a5893a97-deca-4745-bd76-43fefb409f85", "size": 72064701, "created_time": "2019-08-08T15:27:54,226116Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.ms-ims", "checksums": [{"checksum": "ede7e26ae49394ad5c79478c19e534d2bc68a84f1dad8cf4bdb0a56eb87d75f6", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/215/files/primary/AAV9-CG-IP-05.ims"}, "region": "us-east-1"}], "dataset": {"id": "215", "doi": "DOI:10.26275/wlhm-c7ba", "title": "Characterization of adeno associated virus serotypes 4 weeks after pancreas injection in mice", "description": "Assessment of AAV serotypes 6, 8 and 9 for targeting pancreatic innervation.", "abstract": "Adeno-associated virus, expressing mCherry fluorophore under synapsin promoter (AAV-hSyn-mCherry) was injected in the parenchyma of the pancreas. Different serotypes (6, 8, 9) were tested. 4 weeks later, coeliacs were harvested. Tissue clearing and immunohistochemistry staining were applied to identify infected neurons. Confocal microscope was used for imaging acquisition. Analysis and quantification were performed with IMARIS software."}}