{"id": "package:ad740d56-7fe0-4bd1-9d46-c15054514342", "name": "iWAT34_VLS_THch1_CGRPch2_GFPch3.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/ad740d56-7fe0-4bd1-9d46-c15054514342", "size": 20126383248, "created_time": "2021-07-12T19:31:14,876123Z", "updated_time": "2021-07-12T19:47:33,936566Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "428895078baf6dcaecb43258f86382e9e50141afcc0d95d21fb887c2666dd462", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT34/sam-iWAT34/iWAT34_VLS_THch1_CGRPch2_GFPch3.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}