{"id": "package:b248ee2a-d318-4b30-a548-93f40f3d727d", "name": "AAV9andnNOS pC MP M228 20XZ 210106_210118_5.jpx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/b248ee2a-d318-4b30-a548-93f40f3d727d", "size": 1814339, "created_time": "2024-02-15T03:08:37,891339Z", "updated_time": "2024-02-15T03:12:12,044249Z", "version": "1", "mime_type": "image/jpx", "checksums": [{"checksum": "8f2044475d0e141aac8d702f9ffd6bd5", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/386/files/derivative/sub-228/sam-M228/AAV9andnNOS pC MP M228 20XZ 210106_210118_5.jpx"}, "region": "us-east-1"}], "dataset": {"id": "386", "doi": "DOI:10.26275/pc5x-w5l3", "title": "Transduction of systemically administered adeno-associated virus in the colonic enteric nervous system of adult mice", "description": "This dataset contains photomicrographs of the systemic AAV mouse colon enteric nervous system (ENS) that were acquired using confocal microscopes, visualized in three dimensions, and digitally segmented.", "abstract": "To map and characterize the distribution patterns, segmental differences, and morphology and neurochemical identity of transduced cells in the colon of adult mice following systemic administration of adeno-associated virus (AAV)9 variants. We used AAV9-CAG-GFP (AAV9), AAV-PHP.S-hSyn1-tdTomato farnesylated (PHP.S-tdTf) and some other variants to investigate the segmental distribution, morphologies and neurochemical coding of the AAV transduction. The vectors were retro-orbitally injected in male and female adult C57BL/6J, ChAT-Cre and NOS1-Cre mice, and three weeks later, the colon was prepared for microcopy with or without immunohistochemistry for neuronal and non-neuronal markers, including the pan-neuronal markers: HuC/D, protein gene product (PGP) 9.5 and NeuN; the markers of the most representative excitatory and inhibitory motor and sensory neurotransmitters: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS) and calbindin; nerve fibers: calcitonin gene related peptide alpha (αCGRP), tyrosine hydroxylase (TH), and vasoactive intestinal peptide (VIP); glial cells: glial fibrillary acidic protein (GFAP), and S100 calcium-binding protein B (S100B); macrophage: ionized calcium binding adaptor molecule 1 (Iba1) and the interstitial cells of Cajal (ICC): c-Kit (a proto-oncogene encoding the receptor tyrosine kinase protein, a.k.a. CD117). Photomicrographs were acquired in confocal microscopes, visualized in three dimensions and segmented digitally. The data demonstrate the segmental and regional differences of neurons and nerve fibers transduced by systemic delivery of AAV9 and its modified types in the adult mouse colon, contrasting with previous reports using delivery in neonatal and juvenile mice. The segmental difference with a decrease in the transduction by all AAV variants in the transverse colon and almost none in the distal colon. In the proximal colon, the AAV9-transduced myenteric neurons were unevenly distributed in the myenteric plexus. The transduction was higher in cholinergic (ChAT) than inhibitory (nNOS) or sensory (calbindin) myenteric neurons, while there was no transduction in enteric glia that were located among the AAV9 and/or AAV-PHP.S-tdTf transduced neurons and nerve fibers. Iba1 positive macrophages with various morphologies did not show increase in different layers. AAV9/c-Kit data can be found in a published manuscript and a metadata set on SPARC Science portal."}}