{"id": "package:b2bf6ee1-e41c-4539-9bf5-fe7ac26e87f5", "name": "PGP9.5_TBS_20150212_SMP.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/b2bf6ee1-e41c-4539-9bf5-fe7ac26e87f5", "size": 2217079, "created_time": "2022-02-22T21:26:39,623238Z", "updated_time": "2022-02-22T21:26:40,042752Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "07903fe841232075ffc0add3b9e55e90175f8992f70849610550ca4d7b089cef", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/216/files/derivative/sub-20150212P/sam-PGP9-5-20150212P/PGP9.5_TBS_20150212_SMP.tif"}, "region": "us-east-1"}], "dataset": {"id": "216", "doi": "DOI:10.26275/of13-iokw", "title": "Antibodies tested in the colon - Pig", "description": "List of antibodies that were tested in the pig colon. Information on source, species, dilution, and whether the test was performed on whole colonic wall with CLARITY, or whole mount preparations of submucosal and myenteric plexus.", "abstract": "To establish a table listing antibodies that we tested and characterized to label enteric neurons and/or nerve fibers, enteric glial cells and interstitial cells of Cajal (ICC) in the pig colon. The table will serve as reference to assess the suitability of antibodies to be used in immunostaining with whole mount preparations of enteric plexus, CLARITY cleared intact samples and/or cryostat sections of pig colon. The pig colon samples were collected from proximal segment and prepared as (1) whole mounts of the submucosal layer, and of myenteric and longitudinal muscle layers, (2) whole colonic wall cleared by passive CLARITY technique (PACT), or cryostat sections (8-10 µm). Immunofluorescence with these antibodies listed in the table was applied to label the various neuronal subpopulations, enteric glial cells and interstitial cells of Cajal.  Double immunostaining was used to determine the co-localization of different markers. Photomicrographs were acquired with confocal microscopes or epifluorescence microscopes. Remarks and notes about the antibodies are briefly described and some representative figures are assembled in a file for preview of immunofluorescent staining. The dataset provides information on primary antibodies tested in the pig colon, which has been less studied compared with the colon of other species. In addition, the compatibility of immunolabeling using these antibodies with passive CLARITY technique has never been reported so far."}}