{"id": "package:b4863787-cf66-44b0-8412-0ddbfb0fcda6", "name": "iWAT57_PRVGFP_VLS_LS_ZF063_THch1_GFPch2.ims", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/b4863787-cf66-44b0-8412-0ddbfb0fcda6", "size": 8403109153, "created_time": "2019-12-11T11:58:21,562322Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.ms-ims", "checksums": [{"checksum": "40530909e13b254d12a0d1750d89f07154e9640fc154b325952ae962d58a06ed", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/primary/sub-iWAT57/sam-iWAT57/iWAT57_PRVGFP_VLS_LS_ZF063_THch1_GFPch2.ims"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}