{"id": "package:bb2a4258-ac4d-41e4-a5d7-b3dea9b6b3f0", "name": "sam-FS85004888_ch2-2019_02_07_02_FS85004888_Sigma.xlsx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/bb2a4258-ac4d-41e4-a5d7-b3dea9b6b3f0", "size": 2389707, "created_time": "2023-07-21T23:45:14,975977Z", "updated_time": "2023-07-21T23:46:07,674366Z", "version": "1", "mime_type": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "checksums": [{"checksum": "de80aad878322eea0a077f3ac26f8f5e", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/330/files/primary/sub-FS85004888/sam-FS85004888-Ch2/sam-FS85004888_ch2-2019_02_07_02_FS85004888_Sigma.xlsx"}, "region": "us-east-1"}], "dataset": {"id": "330", "doi": "DOI:10.26275/pa9i-likc", "title": "Ussing chamber experiments for distension evoked secretion in human colon", "description": "Distention evoked secretion in human colon", "abstract": "Based on different animal models, a correlation between motility and secretion in the gastrointestinal tract has been established previously. Within the aim of this work, new insights into the distension- and pressure-induced secretion were gained by means of the voltage clamp method in the Ussing chamber. For this purpose, experiments were also performed for the first time on human intestinal resections. This dataset contains the results of Ussing chamber experiments for distension-evoked secretion in mucosa/submucosa preparations from human colon. Distension volume and pressure were precisely controlled by a custom injection system, and we used a pharmacological approach to identify ion channels and pathways that are involved in the secretory responses. It was shown that distension elicited a secretory response in human intestinal samples; chloride and bicarbonate anions mediate the distension-induced secretion evident by the significant reduction of distension-induced secretion in chloride- and chloride/bicarbonate-free experimental buffers. Therefore, the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) plays a major role in these processes in humans. Reduction of distension by increasing compression via incorporation of filters showed that the pressure-induced secretory response is mediated primarily by distension rather than compression. In addition, higher pressures of 2-60 mmHg also elicited higher secretory responses in human tissues. In four of five human large-bowel regions tested, there was a lower distension-induced secretion response with mucosal stimulation compared to serosal stimulation. Because the cyclooxygenase inhibitor piroxicam significantly reduced the distension-induced secretion response, an important role of prostaglandins in distension-induced secretion was revealed. Subsequent application of tetrodotoxin revealed a minor but significant nervous component. The ꙍ-conotoxin GVIA-sensitivity demonstrated the importance of mechanosensitive synapses in mediating distension-induced secretion in the human colon. Thus, the results of this work demonstrate that distension-induced secretion in humans is mediated by both prostaglandins and nerves."}}