{"id": "package:be8b0331-4d98-4f16-af81-76fa4b92d424", "name": "distal_2.json", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/be8b0331-4d98-4f16-af81-76fa4b92d424", "size": 17450395, "created_time": "2021-10-21T01:15:47,303017Z", "updated_time": "2021-10-21T01:15:48,966537Z", "version": "6", "mime_type": "application/json", "checksums": [{"checksum": "e88e7fe62a833e90042774e8d9d7d55f0e8aa2ff009cc4380b490ae597b7fff7", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/34/files/derivative/Scaffolds/sub-all_direct-stim_transverse-colon/distal_2.json"}, "region": "us-east-1"}], "dataset": {"id": "34", "doi": "DOI:10.26275/6lqa-ghyr", "title": "Influence of direct colon tissue electrical stimulation on colonic motility in anesthetized male Yucatan minipig", "description": "Direct proximal colon, transverse colon and colorectum wall electrical stimulation; monitor colon wall bio-impedance and colon manometry in all regions (proximal, transverse and distal) before, during and post stimulation.", "abstract": "Background: Neuromodulation is emerging as an alternative therapy to bowel dysfunctions. However, knowledge on optimal stimulation modalities is lacking. Aim: Map the colonic wall impedance and luminal pressure changes to electrical stimulation of the colon and autonomic nerves in a porcine model. Methods: Anesthetized male Yucatan pigs (27-32 kg) were used. In a first set, direct electrical stimulation of proximal (pC), transverse (tC) and distal (dC) colon was done using planar flexible multi-electrode array panels. Different stimulation parameters for single or multiple electrical stimulations at 2-100 Hz, 2-4 msec, 4-15 mA paradigm were applied.  In all instances, pC, tC and dC wall bioimpedance and luminal pressure (manometry) changes were monitored before, during and after stimulation. Regional colonic motility index was quantified by measuring the area under the curve of the phasic component of the luminal pressure trace (pAUC). Colonic luminal pressure map was generated. Results: Direct pC, tC and dC stimulations caused visible local circular (segmental-like in proximal and transverse colon) or longitudinal muscle contractions (distal colon) that correlated with respective bio-impedance changes. Optimal contractile response was observed at 10 Hz, 2 ms, 15mA. It increased luminal pressure changes in pC, tC and dC (143.0 ± 40.7 %, 135.8 ± 59.7% and 142.0 ± 62% respectively).   Conclusions: Direct colon wall electrical stimulation causes primarily robust local contractions but also modulates  distant colonic regions motility. File format and size: Manometry data were collected using Spike 2 and are available as .smr and .s2r files. For each pig  which underwent direct colon tissue stimulation, Baseline lasted 1800 s (30 min); Stimulation lasted 900 s (15 min); Post-stimulation lasted 1800 s (30 min). There are up to 12 channels for each pig. (N.B.: Some channels may not be available (for some pigs as the\nprobes stopped working properly during the experiment.) Channels are labeled as follow (P:proximal, T:transverse, D: distal): P10, P13, P16, P19; T10, T13, T16, T19; D10, D13, D16, D19. The data were extracted using a Spike 2 script code and inserted into an excel file for further analysis. Bioimpedance data were collected using and are available in .pl2 format. The data were extracted using a Matlab script code and inserted into an excel file for further analysis.                                                                                                          Files Numbers: \nDirect proximal colon stimulation:  Pig #27,28,29,30,31,32,33,41,42,43,44,45,46,47\nDirect transverse colon stimulation: Pig# 18,20,21,22,23,24,25,26\nDirect distal colon stimulation: Pig # 48, 50, 51, 52, 53,54"}}