{"id": "package:be9c5a7e-a16a-4742-882a-4e911fb135b9", "name": "iWAT38_DUS_LS_ZF063_THch1_GFPch2_CD31ch3.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/be9c5a7e-a16a-4742-882a-4e911fb135b9", "size": 17805756500, "created_time": "2021-07-14T14:39:17,075151Z", "updated_time": "2021-07-14T14:53:56,631286Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "4155732a2916304abff21dfa5fc0a4b5fe9b17d51ea50ec3df83994d23c1b679", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/175/files/derivative/sub-iWAT38/sam-iWAT38/iWAT38_DUS_LS_ZF063_THch1_GFPch2_CD31ch3.tif"}, "region": "us-east-1"}], "dataset": {"id": "175", "doi": "DOI:10.26275/mhq6-csy1", "title": "iWAT (inguinal white adipose tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "We utilize pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iWAT in the mouse. Types of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts Techniques used: Viral injection of pseudorabies virus into iWAT, iDISCO, Light sheet microscopy, Confocal microscopy Formats: Excel, Word, Imaris image file, TIF file."}}