{"id": "package:c601e9e2-8718-4b98-80a9-d9af5aa9e72b", "name": "sam-rat89-corp-3_ses-nnos488hu594-zstack3-size.jpx", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/c601e9e2-8718-4b98-80a9-d9af5aa9e72b", "size": 7556206, "created_time": "2023-08-10T16:26:11,898699Z", "updated_time": "2024-12-20T14:15:03,567042Z", "version": "1", "mime_type": "image/jpx", "checksums": [{"checksum": "59e818a41319753b6a2cb3f9e1293e7f1ad255447dcc48c3caf87c2f340ac92b", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/366/files/derivative/sub-rat89/sam-rat89-corp-3/sam-rat89-corp-3_ses-nnos488hu594-zstack3-size.jpx"}, "region": "us-east-1"}], "dataset": {"id": "366", "doi": "DOI:10.26275/mhji-cn6n", "title": "Morphologies dimensions and targets of gastric nitric oxide synthase neurons in the rat stomach", "description": "This dataset contains images and analysis used to quantify the distributions and targets of neuronal nitric oxide synthase (nNOS) immunoreactive neurons in the rat stomach.", "abstract": "To investigate the distributions and targets of neuronal nitric oxide synthase (nNOS) neurons in the muscle layers of the rat stomach in order to better understand how these neurons influence stomach movements. This dataset contains images and analysis used to quantify the distributions and targets of neuronal nitric oxide synthase (nNOS) immunoreactive neurons in the rat stomach. These data show that nitrergic neurons make up about 30% of all myenteric neurons in all gastric regions, with a small population appearing in the submucosal ganglia. The myenteric cell bodies had single axons, Type I morphology, and a wide range of sizes. Five targets were identified: the longitudinal, circular, and oblique layers of the external muscle, the muscularis mucosae, and arteries within the gastric wall. There was also a dense innervation of the pyloric sphincter. Both nNOS immunohistochemistry and NADPHd histochemistry showed that nitrergic neurons did not provide baskets of terminals around myenteric neurons, which suggests gastric interneurons do not express nNOS, unlike intestinal interneurons. The high densities of nNOS fibers throughout the CM and the pyloric sphincter suggest there is highly regulated, fine graded inhibitory control of motor function in these regions."}}