{"id": "package:d0a7d434-24ee-48c8-a74c-e0ef0107344a", "name": "CTB-IP-1W-L13-2.ims", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/d0a7d434-24ee-48c8-a74c-e0ef0107344a", "size": 980960, "created_time": "2019-10-07T17:33:03,731301Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.ms-ims", "checksums": [{"checksum": "91d5e27d011a0414ef23767311595c223949d64a96cb79c417d266377595bce6", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/57/files/source/DRG L13/CTB-IP-1W-L13-2.ims"}, "region": "us-east-1"}], "dataset": {"id": "57", "doi": "DOI:10.26275/xmsp-wwtu", "title": "Quantification of Cholera Toxin Subunit Beta (CTb) positive neurons in the coeliac nodose and dorsal root ganglia 1 week after pancreas injection in mice", "description": "Quantification of CTB in coeliac, nodose, and dorsal root ganglia 1 week after intrapancreatic injection", "abstract": "The neuronal tracer Cholera Toxin Subunit Beta (CTb) conjugated with a fluorophore (AlexaFluor 488) is injected in the parenchyma of the pancreas. 1 week later neuronal ganglia, including Nodoses, Coeliac and thoracic Dorsal Root Ganglia are harvested. Tissue clearing and staining for CTb is performed using Idisco protocol. Image acquisition is done utilizing a confocal microscope. Further, quantification of CTb positive neurons is assessed using IMARIS software."}}