{"id": "package:dd559f7e-af7c-490a-9e45-d43daac0574b", "name": "sam-20210818-L2-shear-1.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/dd559f7e-af7c-490a-9e45-d43daac0574b", "size": 2177546308, "created_time": "2022-06-13T20:32:07,947640Z", "updated_time": "2022-08-27T01:50:48,283376Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "bb17147f7d16c7c5155733d42c5f6eab023e51af707cb35da13d6df6c89a2bd3", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/302/files/primary/sub-20210818/sam-20210818/perf-20210818-L2-shear/sam-20210818-L2-shear-1.tif"}, "region": "us-east-1"}], "dataset": {"id": "302", "doi": "DOI:10.26275/kgkj-6vb9", "title": "Calcium imaging of mouse dorsal root ganglion (DRG) neurons in response to chemical stimuli of distal colon and rectum (colorectum)", "description": "GCaMP recordings from thoracolumbar (T12 to L2) and lumbosacral (L5 to S1) DRG in responses to two chemical stimuli to the attached colorectum, i.e., acidic hypertonic solution (AHS) and inflammatory soup (IS).", "abstract": "The objective of this study is to systematically characterize the chemosensitivity of colorectal afferents to osmotic (AHS) and inflammatory stimuli (IS) and determine their topological locations in the thoracolumbar and lumbosacral DRG. The dataset consists of GCaMP6f image recordings of functional responses of DRG neurons to chemical stimuli at the attached colorectum. Image recordings captures one entire DRG at 60 frames per sec with single-neuron resolution. DRG neural activities were first evoked by mechanical distension (15, 30, 45 and 60 mmHg) followed by perfusion of chemicals (AHS or IS) through the lumen of the attached colorectum. There is a significant overlapping of mechanosensitive and chemosensitive sensory neurons innervating the colorectum."}}