{"id": "package:de189ebb-0d81-4d92-abfb-3ee513acd006", "name": "LA Middle Atrium.tif", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/de189ebb-0d81-4d92-abfb-3ee513acd006", "size": 21151106, "created_time": "2024-01-04T19:54:00,587150Z", "updated_time": "2024-01-05T19:48:51,808982Z", "version": "1", "mime_type": "image/tiff", "checksums": [{"checksum": "cca3d3e19fd8ce8c8977dabedb7c0e7b", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/373/files/primary/sub-H182/sam-2/LA Middle Atrium.tif"}, "region": "us-east-1"}], "dataset": {"id": "373", "doi": "DOI:10.26275/86ve-meck", "title": "Distribution and morphology of calcitonin gene-related peptide (CGRP) innervation in flat mounts of whole rat atria and ventricles", "description": "The sensory (nociceptive) innervation of the whole rat heart (including the atria and ventricles) was immunohistochemically labeled for CGRP. Moreover, high-quality images of the whole tissue were acquired at the single-cell/axon/varicosity resolution.", "abstract": "Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. To address this, we prepared whole rat heart tissues as flat-mounts, labeled them for CGRP, and acquired high quality images of the whole tissues. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls and the walls of the great vessels including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and interventricular septum."}}