{"id": "package:e68135a3-40af-4acd-9732-e6fda7a435f2", "name": "SPARC_BAT92_Ventral_Upper_Spine.ims", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/e68135a3-40af-4acd-9732-e6fda7a435f2", "size": 2945282465, "created_time": "2020-01-23T15:36:55,836290Z", "updated_time": "2022-12-13T15:43:03,032359Z", "version": "1", "mime_type": "application/vnd.ms-ims", "checksums": [{"checksum": "089782fa1b51a1987106d334e87b7c7a7834deea92e5fc1fa102a259b4485968", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/73/files/primary/sub-iBAT92/Imaris Images/SPARC_BAT92_Ventral_Upper_Spine.ims"}, "region": "us-east-1"}], "dataset": {"id": "73", "doi": "DOI:10.26275/xkoa-oqec", "title": "iBAT (interscapular Brown Adipose Tissue) sympathetic innervation circuit pseudorabies viral tracing in reporter mice", "description": "Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.", "abstract": "We utilize pesudorabies virus (PRV) retrograde tracing in combination with reporter mice, iDISCO, and confocal/light sheet microscopy to identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the iBAT in the mouse. \nTypes of data collected: High resolution image files, qualitative anatomical identifcation, quantitative cell counts\nTechniques used: Viral injection of pseudorabies virus into iBAT, iDISCO, Light sheet microscopy, Confocal microscopy"}}