{"id": "package:eeda8d06-cf5a-469d-8a4c-325a4b615ef8", "name": "RM2 rat IVS CGRP 488 20x-05_AcquisitionBlock4_pt4-ApoTome deconvolution-35.czi", "self_uri": "https://services.scicrunch.io/sparc/drs/v1/objects/eeda8d06-cf5a-469d-8a4c-325a4b615ef8", "size": 578043136, "created_time": "2024-01-09T17:49:01,427235Z", "updated_time": "2024-01-09T18:43:28,037511Z", "version": "1", "mime_type": "image/x.vnd.zeiss.czi", "checksums": [{"checksum": "cc0341ff419fc359edae5140933262e95a8ff850affb787401a6c2506461f86a", "type": "sha256"}], "access_methods": [{"type": "s3", "access_url": {"url": "s3://prd-sparc-discover50-use1/373/files/source/sub-RM2/sam-5/montage/RM2 rat IVS CGRP 488 20x-05_AcquisitionBlock4_pt4-ApoTome deconvolution-35.czi"}, "region": "us-east-1"}], "dataset": {"id": "373", "doi": "DOI:10.26275/86ve-meck", "title": "Distribution and morphology of calcitonin gene-related peptide (CGRP) innervation in flat mounts of whole rat atria and ventricles", "description": "The sensory (nociceptive) innervation of the whole rat heart (including the atria and ventricles) was immunohistochemically labeled for CGRP. Moreover, high-quality images of the whole tissue were acquired at the single-cell/axon/varicosity resolution.", "abstract": "Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. To address this, we prepared whole rat heart tissues as flat-mounts, labeled them for CGRP, and acquired high quality images of the whole tissues. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls and the walls of the great vessels including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and interventricular septum."}}